BD LSR II User’s Guide bdbiosciences.com Part No. 642221 Rev A June 2007 BD Biosciences 2350 Qume Drive San Jose, CA 95131-1807 USA Tel (877) 232-8995 Fax (800) 325-9637 facservice@bd.com Asia Pacific Tel (65) 6-861-0633 Fax (65) 6-860-1593 Brazil Tel (55) 11-5185-9995 Fax (55) 11-5185-9895 Canada Toll Free (888) 259-0187 Tel (905) 542-8028 Fax (888) 229-9918 canada@bd.com Europe Tel (32) 2 400 98 95 Fax (32) 2 401 70 94 help.biosciences@europe.bd.com Japan Nippon Becton Dickinson Company, Ltd. Toll Free 0120-8555-90 Tel 81-24-593-5405 Fax 81-24-593-5761 Mexico Toll Free 01-800-236-2543 Tel (52) 55 5999 8296 Fax (52) 55 5999 8288 © 2007, Becton, Dickinson and Company. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, stored in retrieval systems, or translated into any language or computer language, in any form or by any means: electronic, mechanical, magnetic, optical, chemical, manual, or otherwise, without prior written permission from BD Biosciences. The information in this guide is subject to change without notice. BD Biosciences reserves the right to change its products and services at any time to incorporate the latest technological developments. Although this guide has been prepared with every precaution to ensure accuracy, BD Biosciences assumes no liability for any errors or omissions, nor for any damages resulting from the application or use of this information. BD Biosciences welcomes customer input on corrections and suggestions for improvement. BD, BD logo and all other trademarks are property of Becton, Dickinson and Company. © 2007 BD Texas Red™ and Pacific Blue™ are trademarks, and Cascade Blue® and Alexa Fluor® are registered trademarks of Molecular Probes, Inc. Sapphire and Radius are trademarks and Coherent is a registered trademark of COHERENT, INC. ALPHA is a trademark of Omega Optical, Inc. SPHERO is a trademark of Spherotech, Inc. Xcyte is a trademark of Lightwave Electronics. Microsoft and Windows are registered trademarks of Microsoft Corporation. Teflon is a registered trademark of E.I. du Pont de Nemours and Company. JDS Uniphase is a trademark of JDS Uniphase, Inc. ALPHA is a trademark of Omega Optical, Inc. All other company and product names might be trademarks of the respective companies with which they are associated. Patents BD LSR II is covered by one or more of the following US patents and foreign equivalents: 4,745,285; 4,844,610; and 6,014,904. PerCP: US 4,876,190 APC-Cy7: US 5,714,386 BD FACS lysing solution: US 4,654,312; 4,902,613; and 5, 098,849 FCC Information WARNING: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment. NOTICE: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his or her own expense. Shielded cables must be used with this unit to ensure compliance with the Class A FCC limits. This Class A digital apparatus meets all requirements of the Canadian Interference-Causing Equipment Regulations. Cet appareil numérique de la classe A respecte toutes les exigences du Réglement sur the matériel brouilleur du Canada. History Revision Date Change Made 334717 Rev. A 12/02 Initial release 338639 Rev. A 10/04 Updated software terminology and screen shots for BD FACSDiva software version 4.1 640752 Rev. A 5/06 Updated software terminology and screen shots for BD FACSDiva software version 5.0 642221 Rev A 6/07 Updated software terminology and screen shots for BD FACSDiva software version 6.0 Contents About This Guide xi Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi BD LSR II Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xii BD LSR II Online Help . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xii Printed Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii Electronic Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiv Technical Assistance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv Safety and Limitations xvii Laser Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii Laser Product Classification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xviii Precautions for Safe Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xviii Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xix Biological Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xx General Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxi Symbols and Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxi Limitations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxiii Chapter 1: Introduction 25 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 Power Switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 Handles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27